Bankpeptide routinely synthesizes biotinylated peptides for use in protein-protein interaction studies. Although biotin can be introduced either N- or C-terminally (via lysine residues), we recommend the use of an N-terminal modification owing to its low cost, higher success rate, shorter turnaround time, and ease of operation. Peptides are synthesized from the C-terminus to the N-terminus; therefore, the N-terminal modification is the last step in the SPPS protocol, and no additional specific coupling steps are required. In contrast, a C-terminal modification requires additional steps, and is usually more complex. However, in principle the biotin can be positioned anywhere.
Biotin can be separated from the peptide by a variety of different linkers or spacers. Nevertheless, it is recommended that a flexible spacer such as Ahx (a 6-carbon linker) is included to render the biotin label more stable or flexible.
Bankpeptide offers biotinylation at either the N- or C-terminus: biotin-N-terminus, Lys-biotin-middle of the peptide, and Lys-biotin-C-terminus.
We also offer biotinylation using an Ahx linker or a long carbon (LC) linker: biotin-Ahx-Nterminus, Lys-Ahx-biotin-middle of the peptide, Lys-Ahx-biotin-C-terminus.
What is the minimum quantity for a peptide?
The minimun for one peptide should be 1mg, and there is no maximum quantity for a peptide at Bankpeptide.
Which kind of solvent should I use?
According to your needs, the peptides can often dissolved in water, DMF,DMSO, and PBS Buffer.
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MD2 mediates angiotensin II-induced cardiac inflammation and remodeling via directly binding
To Ang II and activating TLR4/NF-κB signaling pathway,Basic Research in Cardiology,Weijian Huang.
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